S-Probes* are the newest tip design for Plexon probes. They are also the strongest. The entire tip is solid stainless steel and shaped similar to a sharpened pencil. It is not recommended to use any Plexon probe to puncture dura, however, those customers who must use the probe to puncture dura may have the best success using the S-Probe tip.
Flexibility in the manufacturing process allows for different electrode site arrangements and spacing, as well as optional fluid delivery channels for precise drug delivery or fiber optic lines for optogenetic stimulation intermixed within the recording sites. The precise linear arrangement of the electrode sites enables current source density analysis of the field potential signals as recorded from laminar neural structures.
The platinum/iridium electrode recording sites are available in 15, 20, 25 and 40µm diameter options. The small electrode site diameter (15µm) enables effective single-unit recording, while a unique etching process of the electrode surface results in lowered impedances without significantly changing the overall surface area of the exposed recording site. The result is a small site suitable for resolving single units coupled with low impedance for a better signal-to-noise ratio.
S-Probes are conveniently available in single, stereotrode and tetrode configurations, each with a choice of 8, 16, 24, or 32 channels.
Below are all three 16 channel configurations illustrated with four fluid capillaries or optic fibers:
S-Probes are Plexon’s newest product in the electrodes, probes and arrays category. S-Probes are expertly handcrafted and made to order with many finished options available. As a result, please expect a six week manufacturing lead time for standard specifications. Some specifications or configurations, such as tetrodes, can take an additional two to three weeks. Your Plexon Sales Engineer is happy to provide additional information and to assist you in determining what might be the most advantageous S-Probe configuration.
S-Probes are customizable, as can be seen from the table below. It may also be possible to produce probes beyond the specifications below. As a result, it is recommended that you discuss your needs with a Plexon Sales Engineer to determine the best configuration and if your unique request can be accommodated.
|Feature||Specifications and Options||Remarks|
|Application||In vivo; acute|
|Channel counts||8, 16 24, or 32|
|Total probe length||30 to 150mm||Length is customizable within range provided; however, the most typical request is 100mm. Total probe length is measured from tip to the connector. The difference between the total probe length and the reinforcement tube length is a minimum of 25mm.|
|Probe OD||185 to 360μm||Probe diameters vary based on the number of electrodes, fluid channels and optic fibers. See the Probe diameter tables found in the Probe Technical Guide under Technical Specifications.|
|Reinforcement tube length||5 to 125mm||Length is customizable within range provided|
|Reinforcement tube outer diameters||460 or 640μm||Probe diameters of 236μm and greater require a 640μm reinforcement tube|
|Electrode construction||15μm Pt/Ir electrode site diameter, circular shape, HML insulated (polyimide), and secured in medical-grade epoxy||15μm diameter sites are recommended for resolving single units (action potentials).|
|Electrode configurations||Single, stereotrode or tetrode||Single configuration is most typical; stereotrodes and tetrodes tend to be used in areas with an especially high density of neurons (i.e. hippocampus).|
|Inter-electrode spacing||50μm, 75μm, 100μm, 150μm, or 200μm along length of probe;
50μm within stereotrode or tetrode group
|Distance from tip to the closest electrode site||
Dependant on probe diameter (See table below):
|The numbering of the electrode sites is such that channel #1 is closest to the connector. Conversely, the electrode site closest to the tip is the largest channel # on the probe.|
|Stimulation options||Single: Up to four fluid capillaries and/or optic fibers
Stereotrode or Tetrode: Up to two fluid capillaries and optic fibers
|Single: Able to accomodate any combination of up to four additional fluid capillaries/optic fibers. Examples: four fluid capillaries; or four optic fibers; or one fluid capillary with three optic fibers. The total count of fluid capillaries plus optic fibers may not exceed four.
Stereotrode or Tetrode: Able to accomodate any combination of up to two additional fluid capillaries/optic fibers. The same examples that apply for the single configuration above apply here, however, the total count of fluid capillaries plus optic fibers may not exceed two.
|Fluid capillary ID||40μm||Fluid capillaries are slightly offset from the center line of the electrodes.|
|Fluid capillary OD||60μm|
|Optic fiber OD||50μm||Optic fibers are slightly offset from the center line of the electrodes.|
8 channel: CON/8o50m-10P
16 channel: 2x CON/8o50m-10P, CON/16m-V or CON/16o25m-18P
24 channel: 3x CON/8o50 or CON/32m-V
32 channel: CON/32m-V
|All are Omnetics connectors.|
|Lifespan||Robust and reusable with a minimum of thirty penetrations, likely many more|
^Batch variation in wire inner diameter may require a larger probe diameter or larger reinforcement tube.
Guides and How To Papers
Technical Specs and Data Sheets