Advice from the Field
What would make the most impact? Type of needle? Motorized controls? Other?
People who do stereotaxic virus surgeries in mice, I'm thinking of upgrading (or replacing) a standard Kopf stereotax for better accuracy of placement and expression. What would make the most impact? Type of needle? Motorized controls? Other?
-Karyn Frick
(@FrickKaryn)
Does anyone have tips for visualizing the location of recording probes for in vivo recordings?
Does anyone have tips for visualizing the location of recording probes for in vivo recordings? We have tried DiI and find that it does not visualize tracts deeper than a few hundred microns. Tips on coating procedures or dyes appreciated!!-Aryn Gittis(@ArynGittis)
Has anyone used the axon targeted gcamp variants in rats for fiber photometry?
Has anyone used the axon targeted gcamp variants in rats for fiber photometry? I tried syn-gcamp8s but had no luck. I've noticed many people have previously used syn-gcamp6s successfully in mice but not sure if there's reason to believe 6s would work better than 8s?— Michael Totty
(@MicTott)
There is a lot of anecdotal information about optogenetic and chemogenetic tools not working, or not working very well, for silencing synaptic terminals.
There is a lot of anecdotal information about optogenetic and chemogenetic tools not working, or not working very well, for silencing synaptic terminals. We decided to try and collect this information to better understand which approaches were tested, and how well they work. 1/3)
— Ofer Yizhar
(@OferYizhar)
Are you doing ephys with optogenetics and are having issues with light-induced artifacts?
Are you doing ephys with optogenetics and are having issues with light-induced artifacts? I learned this great trick from @SteinmetzNeuro, it either completely solves the artifact or greatly reduces it. (1/3)
— Guido Meijer
(@guido_meijer) February 16, 2022
Is there any piece of equipment you really like and recommend?
Systems neuroscientists, is there any piece of equipment you really like and recommend? Nothing too expensive please so don’t say 2P.— Yin lab
(@HenryYin19) February 11, 2022
Could you help my lab get connected with the neuroscience and mental health community?
Hi All, we're new to Twitter. Could you help my lab get connected with the neuroscience and mental health community? Brain stimulation and other neuromodulation techniques, electrophysiology, neuroimaging. We want to hear about it all! Thanks!— Keller Lab @ Stanford
What's something every prospective PhD student should know or read before deciding on joining a lab?
What's something every prospective PhD student should know or read before deciding on joining a lab?— Patrick Mineault
Has anyone done Pavlovian-instrumental transfer (PIT) in mice and have some advice for us in setting this up?
Ahem! I interrupt your regularly scheduled programming complaining about everything that’s wrong in the world to ask a specific science question: Has anyone done Pavlovian-instrumental transfer (PIT) in mice and have some advice for us in setting this up?— Dr. Talia Lerner
How many labs have you worked in?
How many labs have you worked in? (Rotations and internships count!)—Dr. Caroline Bartman
Which animal model is hardest to work on?
Question for all my friends that do brain slice ephys: do you use animals or recorded neurons as replicates in your experiments? We’ve done neurons (report n neurons/n animals), but three journals now have asked that we collapse on animal (average all neurons/animal). Thoughts?— Brady Atwood
Which animal model is hardest to work on?
Which animal model is hardest to work on?— Leor Katz
How can I keep my rat from unplugging himself?
SOS how can I keep my rat from unplugging himself? Lab tape and electrical tape were both a no go.— Jamie Krueger, PhD
(@jamienkrueger) September 8, 2021
Where do you buy your electrodes from?
Neuroscience friends! Who is doing single-unit recordings in mice? Where do you buy your electrodes from? Thanks in advance!
— Dr. Maddie Ray
(@maddiehray) July 19, 2021
Do you conduct your experiments during the animal's light cycle or dark cycle?
Poll for rodent behavioral neuroscientists, esp in learning & memory fields - do you conduct your experiments during the animal’s light cycle or dark cycle? Have you ever tried both and observed a difference in behavioral outcomes?
— Dr Becca, PhD 🐘 (@doc_becca) July 9, 2021
Conversations about Grounds & References
In vivo electrophysiologists. Can we have a conversation about grounds & references? What kind of wire do you use? How do you decide which brain region is a neutral ground? Do you use a reference wire? Why/why not? I use bare steel in cerebellum, ref shorted to ground
— Sam McKenzie (@samamckenzie) January 25, 2021
Tips on Cementing Fiber Stubs to a Rat Skull
Neurotweep hivemind:
— Jonathan Wilson (@jonjwilson) November 13, 2019
Does anybody out there with tips on cementing fiber stubs to rat skull? We're having some issues with stubs coming away from the dental cement. Have tried anchoring with epoxy too, to no avail.
We're using 2.5mm zirconia ferrules with zirconia sleeves.
What software do you use for spike sorting?
what software do you use for spike sorting?
— Justin Kiggins (@neuromusic) September 15, 2017
Light Sources for In Vivo Optogenetics
Hey people doing in vivo opto in mammals, what are you using for light sources these days? I would like to move away from DPSS lasers for my lab's set up.
— Benjamin Saunders (@BenSaunders) August 14, 2018
How long does it take you to produce a full first draft of a paper?
How long does it take you to produce a full first draft of a paper? @AcademicChatter #AcademicTwitter #PhDChat pic.twitter.com/jBEczhTTh3
— Sophia Fedorowicz (@Soph_Fedorowicz) February 8, 2021
Mouse Pellets
Lab question: Bio-Serv’s dustless precision pellets seem to be backordered. Anyone use 20mg pellets (mouse operant chamber) from another company that may be available in different flavors? (That may be able to ship quickly?) Thanks! 🐭
— Lindsay Halladay (@LindsayHalladay) February 9, 2021
How many 1st author papers from postdoc are needed to be considered “productive” “competitive” for a K99 nowadays?
How many 1st author papers from postdoc are needed to be considered “productive” “competitive” for a K99 nowadays? Things have changed since mine in 2011/2012 cycle.
— Dr.KaczorowskiLab (@KaczorowskiLab) April 22, 2021
Favorite Resource for Spiking Data
Neurotwitter: the whole spiking data / single neuron world seems sorta wild, with lots of options for spike sorting, analysis approaches, statistical tests, etc.
— Tom Donoghue (@Tomdonoghue) May 10, 2021
Anyone have favourite resources (guides / tools / method comparisons / formatting recommendations, etc) to recommend?
When you receive AAVs for opto and DREADDs do you standardize the titer?
Neuro twitter AAV users: when you receive AAVs for opto and DREADDs do you standardize the titer? For example, you always use 1×10¹³ vg/mL and if you receive 4.3×10¹³ vg/mL you dilute with sterile PBS.
— Mike McDannald (@mikemcdannald) June 2, 2021
What are your thoughts on robotic stereotaxic instruments (e.g., Neurostar)?
Neurotweeps: What are your thoughts on robotic stereotaxic instruments (e.g., Neurostar)? What is the good, the bad, and the ugly? Anyone using for GRIN lens implants in rats?
— Stephen Maren (@StephenMaren) June 15, 2021
What's the catchphrase from your PhD supervisor that stuck with you?
What's the catchphrase from your PhD supervisor that stuck with you and you hold it in as a lemma?
— Ricardo Henriques (@HenriquesLab) May 11, 2021
If you were start learning Python from a standing start with little programming background, what would you use?
If you were start learning Python from a standing start with little programming background, what would you use?—Gavin Buckingham
(@DrGBuckingham)
How many years did you post doc before you were able to get a TT faculty position?
STEM researchers! How many years did you post doc before you were able to get a TT faculty position? Please also state if you are a woman or are from an underrepresented group (incl LGBTQ2S+)! 8 years, Indigenous woman.—Dr. Melissa Perreault
(@Dr_Perreault)
I’m a PhD student in my 3rd year. I always feel that I’m unable to successfully contribute to academic discussion
Anonymous question "I’m a PhD student in my 3rd year. I always feel that I’m unable to successfully contribute to academic discussion. I rarely have something useful to add or a new idea to raise. When I do, it’s nothing more than a sentence or two. How would you deal with?"
—Academic Chatter
(@AcademicChatter)
If you have an AAV-delivered tool that has verified expression in mice, can you assume that it reliably works in rats?
Folks. If you have an AAV-delivered tool that has verified expression in mice, can you assume that it reliably works in rats? If not, who would you blame? AAV Serotype? Promoter? Tool (opsin/indicator etc?). Thanks!- Ali Mohebi(@mohebial) February 22, 2022
What one tip would you give new-starting PhD students?
What one tip would you give new-starting PhD students?
— Academic Chatter
Does anyone have a good way to batch process @MedAssociates .txt files?
Does anyone have a good way to batch process @MedAssociates .txt files? There must be something more efficient than the MED-PC To Excel program…@JordanYorgason @TheErinCalipari @mattjwanat @DavidBarker_PhD @HowardDopamine @MATLAB
— d_p_cove
(@dp_covey) February 11, 2022
Unsolicited advice for new Beh Neuro
Unsolicited advice for new Beh Neuro PIs. Make an analysis workflow that includes a visual summary of each day's behavioral data. Visualize every day. (1) You will catch issues as they happen. Visualizing data has saved us many times. (2) It is SUPER GRATIFYING.
— Mike McDannald
Best location for a scientific meeting?
Best location for a scientific meeting?— Steve Maren, PhD
Can someone recommend a good Flp-dependent ChR2? (fDIO-ChR2)?
Can someone recommend a good Flp-dependent ChR2? (fDIO-ChR2)?— Aryn Gittis
What sorts of #altac careers are out there for neuro PhDs or perhaps how you found your current job?
I’ve still got time left in grad school but I’ve been thinking about career options both in and outside academia. Anyone have any tips on figuring out what sorts of #altac careers are out there for neuro PhDs or perhaps how you found your current job? DMs also open. Thanks!!—Alex Lamparelli
What is the best way to assess depression-like behavioural phenotypes in mice?
Naive question: What is the best way to assess depression-like behavioural phenotypes in mice? We seem to be moving away from things like forced swim, tail suspension etc. What are the most robust, widely appreciated assays? Bonus if linked to hippocampal neurogenesis.— Paul Frankland
Labs with 3D printers!
Labs with 3D printers!What are the most useful items you've printed? I've looked into head caps, tube holders, behavioral chamber parts, etc. but would love more ideas!— Dr. Maddie Ray
(@maddiehray) September 10, 2021
Dear rat #Electrophysiologists
Dear rat #Electrophysiologists
who use independently-moveable tetrodes, The legend says that some of you screen during sleep (not your sleep, the rat's) - any tips, especially on how to 1) get rats to sleep and 2) not wake them up when you lower the tetrodes?
—El Duvelle
(@ElDuvelle) September 7, 2021
What do you love about your PhD program and what do you think made you a good fit for the school you chose?
I'm applying for PhD programs this fall, and I think this week is the PERFECT week to ask the
community: What do you love about your PhD program and what do you think made you a good fit for the school you chose?
— Nicole T. Quintus (@nicole_quintus) July 27, 2021
Did your university ever provide you with any design training?
Here are a few of the graphics that scientists are regularly asked to make: Visual Abstracts Posters Experiment setups Schematics Presentation slides
— Maayan Harel | Maayan Visuals (@maayanvisuals) June 30, 2021
When building a drive, to connect tetrodes to the EIB (or any connector), what do you do?
Another Q for tetrode #Electrophysiologists:
— El Duvelle (@ElDuvelle) June 28, 2021
When building a drive, to connect tetrodes to the EIB (or any connector), do you:
1) solder tetrode wires to the EIB
2) use the push-pin technique to attach wires to the EIB
I think 1 is more difficult but more reliable... thoughts?
What's the next big thing in Neuroscience?
What’s the next big thing in neuroscience? Transformative paradigm, technique, etc. Serious q
— Cian O'Donnell (@cian_neuro) February 1, 2021
How do you test if you're recording the same neuron over days?
Ephys Twitter, I need your help! How do you test if you're recording the same neuron over days?
— Dr. Emily Aery Jones (@EmilyAeryJones) January 27, 2021
Programs Used to Analyze Electrophysiology Data
Folks who do single unit ephys recordings in freely behaving rats (we use @PlexonNeurosci) what programs do you use to analyze your ephys data. I’m not teaching this fall 🎉 so I have some time to finally (!) learn some coding
— Dr. Susan Sangha (@sanghalab) March 10, 2018
Recruitment Questions to Ask
Upping this fantastic 🧵during recruitment season! I'll add 3 more Qs to ask lab mates:
— Ashwini Ramesh (@Ashwini_Ramesh_) February 5, 2021
1. Is your advisor kind?
2. If you have a professional disagreement with your advisor, how do they react?
3. Following (2), does your advisor continue to treat you kindly?#AcademicChatter https://t.co/x1zeHboZbf
Med Associates Operant Chambers Using Other Software
Has anyone ever run med associates operant chambers using something other than med associates interface and software?
— Reggie Cannady (@CannadyPhD) April 22, 2021
Should journal clubs limit their scope to preprints?
Idea: journal clubs should limit their scope to preprints and post their reviews as public comments on their respective Rxiv preprint server. The more I'm in Science, the more I understand journals as a false and lazy metric Academia uses to misjudge scientist's work.
— PhiloNeuroSci🇺🇲🇬🇾🔬🧠 (@PhiloNeuroSci) April 29, 2021
Standardized Guidelines for Investigating Neural Circuit Control of Behavior in Animal Research
Must read. Awesome job @TheErinCalipari and friends - love the tenor - https://t.co/8z8PH2cYaT
— Alex Shackman (@ajshackman) April 29, 2021
At which distance does stimulation induce most spikes in optogenetic neural stimulation?
when doing optogenetic neural stimulation (e.g. monkey), close to light guide there is most photon flow and it decays with distance. At which distance does stimulation induce most spikes (#neurons*#deltaSpikes)?
— KordingLab 👨💻🧠∇🔬📈,🏋️♂️⛷️🏂🛹🕺⛰️☕🦖 (@KordingLab) June 8, 2021
If you were start learning Python from a standing start with little programming background, what would you use?
If you were start learning Python from a standing start with little programming background, what would you use?—Gavin Buckingham
(@DrGBuckingham)
What would make the most impact? Type of needle? Motorized controls? Other?
People who do stereotaxic virus surgeries in mice, I'm thinking of upgrading (or replacing) a standard Kopf stereotax for better accuracy of placement and expression. What would make the most impact? Type of needle? Motorized controls? Other?
-Karyn Frick
Does anyone have tips for visualizing the location of recording probes for in vivo recordings?
Does anyone have tips for visualizing the location of recording probes for in vivo recordings? We have tried DiI and find that it does not visualize tracts deeper than a few hundred microns. Tips on coating procedures or dyes appreciated!!-Aryn Gittis(@ArynGittis)
I’m a PhD student in my 3rd year. I always feel that I’m unable to successfully contribute to academic discussion
Anonymous question "I’m a PhD student in my 3rd year. I always feel that I’m unable to successfully contribute to academic discussion. I rarely have something useful to add or a new idea to raise. When I do, it’s nothing more than a sentence or two. How would you deal with?"
—Academic Chatter
(@AcademicChatter)
Has anyone used the axon targeted gcamp variants in rats for fiber photometry?
Has anyone used the axon targeted gcamp variants in rats for fiber photometry? I tried syn-gcamp8s but had no luck. I've noticed many people have previously used syn-gcamp6s successfully in mice but not sure if there's reason to believe 6s would work better than 8s?— Michael Totty
(@MicTott)
There is a lot of anecdotal information about optogenetic and chemogenetic tools not working, or not working very well, for silencing synaptic terminals.
There is a lot of anecdotal information about optogenetic and chemogenetic tools not working, or not working very well, for silencing synaptic terminals. We decided to try and collect this information to better understand which approaches were tested, and how well they work. 1/3)
— Ofer Yizhar
(@OferYizhar)
There is a lot of anecdotal information about optogenetic and chemogenetic tools not working, or not working very well, for silencing synaptic terminals.
There is a lot of anecdotal information about optogenetic and chemogenetic tools not working, or not working very well, for silencing synaptic terminals. We decided to try and collect this information to better understand which approaches were tested, and how well they work. 1/3)
— Ofer Yizhar
(@OferYizhar)
What one tip would you give new-starting PhD students?
What one tip would you give new-starting PhD students?— Academic Chatter
Are you doing ephys with optogenetics and are having issues with light-induced artifacts?
Are you doing ephys with optogenetics and are having issues with light-induced artifacts? I learned this great trick from @SteinmetzNeuro, it either completely solves the artifact or greatly reduces it. (1/3)
— Guido Meijer
(@guido_meijer) February 16, 2022
Does anyone have a good way to batch process @MedAssociates .txt files?
Does anyone have a good way to batch process @MedAssociates .txt files? There must be something more efficient than the MED-PC To Excel program…@JordanYorgason @TheErinCalipari @mattjwanat @DavidBarker_PhD @HowardDopamine @MATLAB
— d_p_cove
(@dp_covey) February 11, 2022
Is there any piece of equipment you really like and recommend?
Systems neuroscientists, is there any piece of equipment you really like and recommend? Nothing too expensive please so don’t say 2P.— Yin lab
(@HenryYin19) February 11, 2022
Unsolicited advice for new Beh Neuro
Unsolicited advice for new Beh Neuro PIs. Make an analysis workflow that includes a visual summary of each day's behavioral data. Visualize every day. (1) You will catch issues as they happen. Visualizing data has saved us many times. (2) It is SUPER GRATIFYING.
— Mike McDannald
Could you help my lab get connected with the neuroscience and mental health community?
Hi All, we're new to Twitter. Could you help my lab get connected with the neuroscience and mental health community? Brain stimulation and other neuromodulation techniques, electrophysiology, neuroimaging. We want to hear about it all! Thanks!— Keller Lab @ Stanford
Best location for a scientific meeting?
Best location for a scientific meeting?— Steve Maren, PhD
What's something every prospective PhD student should know or read before deciding on joining a lab?
What's something every prospective PhD student should know or read before deciding on joining a lab?— Patrick Mineault
Can someone recommend a good Flp-dependent ChR2? (fDIO-ChR2)?
Can someone recommend a good Flp-dependent ChR2? (fDIO-ChR2)?— Aryn Gittis
Has anyone done Pavlovian-instrumental transfer (PIT) in mice and have some advice for us in setting this up?
Ahem! I interrupt your regularly scheduled programming complaining about everything that’s wrong in the world to ask a specific science question: Has anyone done Pavlovian-instrumental transfer (PIT) in mice and have some advice for us in setting this up?— Dr. Talia Lerner
How many labs have you worked in?
How many labs have you worked in? (Rotations and internships count!)—Dr. Caroline Bartman
What sorts of #altac careers are out there for neuro PhDs or perhaps how you found your current job?
I’ve still got time left in grad school but I’ve been thinking about career options both in and outside academia. Anyone have any tips on figuring out what sorts of #altac careers are out there for neuro PhDs or perhaps how you found your current job? DMs also open. Thanks!!—Alex Lamparelli
What is the best way to assess depression-like behavioural phenotypes in mice?
Naive question: What is the best way to assess depression-like behavioural phenotypes in mice? We seem to be moving away from things like forced swim, tail suspension etc. What are the most robust, widely appreciated assays? Bonus if linked to hippocampal neurogenesis.— Paul Frankland
Which animal model is hardest to work on?
Question for all my friends that do brain slice ephys: do you use animals or recorded neurons as replicates in your experiments? We’ve done neurons (report n neurons/n animals), but three journals now have asked that we collapse on animal (average all neurons/animal). Thoughts?— Brady Atwood
Which animal model is hardest to work on?
Which animal model is hardest to work on?— Leor Katz
(@Leorkatz)September 30, 2021
Labs with 3D printers!
Labs with 3D printers!What are the most useful items you've printed? I've looked into head caps, tube holders, behavioral chamber parts, etc. but would love more ideas!— Dr. Maddie Ray
How can I keep my rat from unplugging himself?
SOS how can I keep my rat from unplugging himself? Lab tape and electrical tape were both a no go.—Jamie Krueger, PhD
(@jamienkrueger) September 8, 2021
Dear rat #Electrophysiologists
Dear rat #Electrophysiologists
—El Duvelle
(@ElDuvelle) September 7, 2021
What do you love about your PhD program and what do you think made you a good fit for the school you chose?
I'm applying for PhD programs this fall, and I think this week is the PERFECT week to ask the
community: What do you love about your PhD program and what do you think made you a good fit for the school you chose?
— Nicole T. Quintus (@nicole_quintus) July 27, 2021
Where do you buy your electrodes from?
Neuroscience friends! Who is doing single-unit recordings in mice? Where do you buy your electrodes from? Thanks in advance!
— Dr. Maddie Ray
(@maddiehray) July 19, 2021
Did your university ever provide you with any design training?
Here are a few of the graphics that scientists are regularly asked to make: Visual Abstracts Posters Experiment setups Schematics Presentation slides
— Maayan Harel | Maayan Visuals (@maayanvisuals) June 30, 2021
Do you conduct your experiments during the animal's light cycle or dark cycle?
Poll for rodent behavioral neuroscientists, esp in learning & memory fields - do you conduct your experiments during the animal’s light cycle or dark cycle? Have you ever tried both and observed a difference in behavioral outcomes?
— Dr Becca, PhD 🐘 (@doc_becca) July 9, 2021
Conversations about Grounds & References
In vivo electrophysiologists. Can we have a conversation about grounds & references? What kind of wire do you use? How do you decide which brain region is a neutral ground? Do you use a reference wire? Why/why not? I use bare steel in cerebellum, ref shorted to ground
— Sam McKenzie (@samamckenzie) January 25, 2021
What's the next big thing in Neuroscience?
What’s the next big thing in neuroscience? Transformative paradigm, technique, etc. Serious q
— Cian O'Donnell (@cian_neuro) February 1, 2021
Tips on Cementing Fiber Stubs to a Rat Skull
Neurotweep hivemind:
— Jonathan Wilson (@jonjwilson) November 13, 2019
Does anybody out there with tips on cementing fiber stubs to rat skull? We're having some issues with stubs coming away from the dental cement. Have tried anchoring with epoxy too, to no avail.
We're using 2.5mm zirconia ferrules with zirconia sleeves.
How do you test if you're recording the same neuron over days?
Ephys Twitter, I need your help! How do you test if you're recording the same neuron over days?
— Dr. Emily Aery Jones (@EmilyAeryJones) January 27, 2021
What software do you use for spike sorting?
what software do you use for spike sorting?
— Justin Kiggins (@neuromusic) September 15, 2017
Programs Used to Analyze Electrophysiology Data
Folks who do single unit ephys recordings in freely behaving rats (we use @PlexonNeurosci) what programs do you use to analyze your ephys data. I’m not teaching this fall 🎉 so I have some time to finally (!) learn some coding
— Dr. Susan Sangha (@sanghalab) March 10, 2018
Light Sources for In Vivo Optogenetics
Hey people doing in vivo opto in mammals, what are you using for light sources these days? I would like to move away from DPSS lasers for my lab's set up.
— Benjamin Saunders (@BenSaunders) August 14, 2018
Recruitment Questions to Ask
Upping this fantastic 🧵during recruitment season! I'll add 3 more Qs to ask lab mates:
— Ashwini Ramesh (@Ashwini_Ramesh_) February 5, 2021
1. Is your advisor kind?
2. If you have a professional disagreement with your advisor, how do they react?
3. Following (2), does your advisor continue to treat you kindly?#AcademicChatter https://t.co/x1zeHboZbf
How long does it take you to produce a full first draft of a paper?
How long does it take you to produce a full first draft of a paper? @AcademicChatter #AcademicTwitter #PhDChat pic.twitter.com/jBEczhTTh3
— Sophia Fedorowicz (@Soph_Fedorowicz) February 8, 2021
Med Associates Operant Chambers Using Other Software
Has anyone ever run med associates operant chambers using something other than med associates interface and software?
— Reggie Cannady (@CannadyPhD) April 22, 2021
Mouse Pellets
Lab question: Bio-Serv’s dustless precision pellets seem to be backordered. Anyone use 20mg pellets (mouse operant chamber) from another company that may be available in different flavors? (That may be able to ship quickly?) Thanks! 🐭
— Lindsay Halladay (@LindsayHalladay) February 9, 2021
Should journal clubs limit their scope to preprints?
Idea: journal clubs should limit their scope to preprints and post their reviews as public comments on their respective Rxiv preprint server. The more I'm in Science, the more I understand journals as a false and lazy metric Academia uses to misjudge scientist's work.
— PhiloNeuroSci🇺🇲🇬🇾🔬🧠 (@PhiloNeuroSci) April 29, 2021
How many 1st author papers from postdoc are needed to be considered “productive” “competitive” for a K99 nowadays?
How many 1st author papers from postdoc are needed to be considered “productive” “competitive” for a K99 nowadays? Things have changed since mine in 2011/2012 cycle.
— Dr.KaczorowskiLab (@KaczorowskiLab) April 22, 2021
Standardized Guidelines for Investigating Neural Circuit Control of Behavior in Animal Research
Must read. Awesome job @TheErinCalipari and friends - love the tenor - https://t.co/8z8PH2cYaT
— Alex Shackman (@ajshackman) April 29, 2021
Favorite Resource for Spiking Data
Neurotwitter: the whole spiking data / single neuron world seems sorta wild, with lots of options for spike sorting, analysis approaches, statistical tests, etc.
— Tom Donoghue (@Tomdonoghue) May 10, 2021
Anyone have favourite resources (guides / tools / method comparisons / formatting recommendations, etc) to recommend?
At which distance does stimulation induce most spikes in optogenetic neural stimulation?
when doing optogenetic neural stimulation (e.g. monkey), close to light guide there is most photon flow and it decays with distance. At which distance does stimulation induce most spikes (#neurons*#deltaSpikes)?
— KordingLab 👨💻🧠∇🔬📈,🏋️♂️⛷️🏂🛹🕺⛰️☕🦖 (@KordingLab) June 8, 2021
When you receive AAVs for opto and DREADDs do you standardize the titer?
Neuro twitter AAV users: when you receive AAVs for opto and DREADDs do you standardize the titer? For example, you always use 1×10¹³ vg/mL and if you receive 4.3×10¹³ vg/mL you dilute with sterile PBS.
— Mike McDannald (@mikemcdannald) June 2, 2021
What are your thoughts on robotic stereotaxic instruments (e.g., Neurostar)?
Neurotweeps: What are your thoughts on robotic stereotaxic instruments (e.g., Neurostar)? What is the good, the bad, and the ugly? Anyone using for GRIN lens implants in rats?
— Stephen Maren (@StephenMaren) June 15, 2021
What's the catchphrase from your PhD supervisor that stuck with you?
What's the catchphrase from your PhD supervisor that stuck with you and you hold it in as a lemma?
— Ricardo Henriques (@HenriquesLab) May 11, 2021
Follow us on Twitter @PlexonNeurosci