CineLyzer with Single Wavelength Multi-Fiber Photometry System

The single wavelength multi-fiber photometry system is Plexon’s original camera based system that images one end of a branching patch cable containing multiple fibers and measures the photometry signal from the individual fibers in the bundle via image processing techniques. Although new single wavelength systems are no longer sold, support continues.

Features:

  • Record from multiple locations in a single animal or many animals simultaneously with a single system
  • Integrated with video tracking software
  • Camera based
  • GFP based indicators such as GCaMP6
  • RFP based indicators such as R-CaMP2 (optional)
Latest Software Downloads CineLyzer® 4.4.1 Windows 7
                                                      CineDemo 4.4.0

Plexon’s Innovation

By using a CCD camera through a fiber bundle, the channel number can be easily expanded without extra equipment. The single wavelength Multi-Fiber Photometry System is a camera based system that images one end of a patch cable containing a bundle of fibers and measures the photometry signal from the individual fibers in the bundle via image processing techniques. The other end of the patch cable consists of individual fiber branches that are typically connected to fiber stubs implanted into individual brain regions of interest or potentially even implanted into multiple subjects. An excitation light source is projected onto the bundled end of the cable and the excitation light travels down each individual branch to its respective brain region. Fluorescent reporters expressed in each brain region absorb the excitation light and emit an activity dependent light signal that travels back up the fiber branches to be imaged by the sensor.

The diagram  depicts the basic components of CineLyzer with the single wavelength photometry system.    

Coupled With CineLyzer

The single wavelength Multi-Fiber Photometry System is an optional subsystem that is integrated into the CineLyzer behavioral tracking and analysis system. The CineLyzer system provides for digital video recording of the subject along with speed, position, and orientation tracking. It allows you to define arenas and zones and compute statistical information on the subject’s entry into, exit from, or dwell time within these zones or sequences of zones. Dynamic zones centered on the subject can also be used to analyze social interaction between subjects. Thus incorporating photometry into the CineLyzer env