PlexBright Compact LED Optogenetics Modules

At the heart of the PlexBright^® Optogenetic Stimulation System are the cost-effective and powerful PlexBright LED Optogenetics Modules for standard use (Table-top LED Optogenetics Modules), or for use with our PlexBright Commutators for freely behaving animals (Compact or Head-Mounted LED Optogenetics Modules). A full spectrum of wavelengths enables you to get the most out of your research whether you are performing acute or chronic in vivo experiments or in vitro preparations.

LED Optogenetics

Description

Plexon’s second generation, super-intensity PlexBright LED Optogenetics Modules provide outstanding light stability and pulse characteristics – all within a very small footprint. Our ever-expanding series of powerful, fiber-coupled PlexBright LED Modules effectively elicit responses from several opsins targeted in neuroresearch, including channelrhodopsins (ChRs), archaerhodopsins (Arch) and halorhodopsins (NpHR).

PlexBright LED Modules come in a wide range of wavelengths and are capable of emitting continuous, stable, and quick-responding light through the tip of a 200μm core, custom developed optical fiber. PlexBright Compact LED Modules are available in the following seventeen wavelengths: Royal (450nm), Blue (465nm), Green (525nm), Lime (550nm), Yellow (590nm), Orange (620nm), Red (630nm) Crimson (660nm), Infrared 1 (850nm), Infrared 2 (940nm), Near-Infrared 1 (740nm), Near-Infrared 2 (760nm), Near-Infrared 3 (780nm), Near-Infrared 4 (810nm), Ultraviolet 1 (365nm), Ultraviolet 2 (385nm), and Ultraviolet 3 (405nm).

PlexBright Compact LED Optogenetics Modules provide the same performance as the PlexBright Table-top LED Modules, only in an ultra light-weight and miniaturized package designed to be mounted to either of our PlexBright

Commutators that deliver power to the LEDs as they spin freely along with a behaving animal. These modules have recently been enhanced to feature a magnetic connection to the commutator. The new style of magnetic attachment enables faster module interchangeability. These Compact LED Optogenetics Modules are compatible with the PlexBright Dual LED Commutator, PlexBright Dual LED + 16 Channel Commutator for simultaneous neural recording with analog headstages, or the Carousel™ Commutator for simultaneous neural recording with digital headstages.

For maximum power output to the stimulation site, high performance PlexBright Optical Patch Cables have been designed to easily connect to the modules. It is important to note that our PlexBright Optical Patch Cables are LED optogenetics module style-specific.

PlexBright Starter Kits offer the initial products to get started. Kits are available in four experimental designs and with either driver (the economical kit with the LD-1 Single Channel LED Driver or the fully functional kit with the PlexBright 4 Channel Optogenetic Controller with Radiant Software).

In addition to super intensity PlexBright LED Modules, Optical Patch Cables and Commutators, the PlexBright Optogenetic Stimulation System also offers the PlexBright LD-1 Single Channel LED Driver; the more sophisticated PlexBright 4 Channel Optogenetic Controller running Radiant^™ Software; specially designed PlexBright Fiber Stub Implants; the PlexBright Light Measurement Kit; and the PlexBright Optical Fiber Cleaning Kit to safely maintain and extend the life of the fiber tips. If your experiments include freely behaving animals, you may also be interested in learning more about the CinePlex^®and CineLyzer^®Behavioral Research Systems.

Your Plexon Sales Engineer is available to provide additional information and to assist you in determining what configuration might be the best choice for supporting your research.

Technical Specs

Available Wavelengths

Color (Wavelength)	Max Current	Remarks
Royal (450nm)	28.1mW (894mW/mm²)	300mA
Blue (465nm)	24.9mW (792mW/mm²)	300mA	One of the most popular LED modules.
Green (525nm)	7.8mW (249mW/mm²)	300mA	One of the most popular LED modules.
Lime (550nm)	11.5mW (336mW/mm²)	500mA	It offers attractive power and a broad activation spectrum to those targeting halorhodopsins and archerhodopsins.
Yellow (590nm)	3.2mW (102mW/mm²)	250mA	Due to greater power output and spectrum overlap, the Orange (620nm) LED module is often preferred over the Yellow (590nm).
Orange (620nm)	11.0mW (349mW/mm²)	250mA
Red (630nm)	11.8mW (375mW/mm²)	1000mA
Crimson (660nm)	15.1mW (476mW/mm²)	1000mA
Infrared 1 (850nm)	13.2mW (422mW/mm²)	1000mA
Infrared 2 (940nm)	14.0mW (445mW/mm²)	1000mA
Near-Infrared 1 (740nm)	8.5mW (270mW/mm²)	1000mA	Near-Infrared LED modules are used for photothermal inhibition of neural activity
Near-Infrared 2 (760nm)	11.2mW (356mW/mm²)	1000mA	Near-Infrared LED modules are used for photothermal inhibition of neural activity
Near-Infrared 3 (780nm)	7.2mW (228mW/mm²)	1000mA	Near-Infrared LED modules are used for photothermal inhibition of neural activity
Near-Infrared 4 (810nm)	8.4mW (268mW/mm²)	1000mA	Near-Infrared LED modules are used for photothermal inhibition of neural activity
Ultraviolet 1 (365nm)	13.5mW (430mW/mm²)	1000mA	Ultraviolet LED modules for Ca indicator and Light-regulated GABAA receptor (LIGABAR)
Ultraviolet 2 (385nm)	26.0mW (829mW/mm²)	1000mA	Ultraviolet LED modules for Ca indicator and Light-regulated GABAA receptor (LIGABAR)
Ultraviolet 3 (405nm)	27.2mW (865mW/mm²)	1000mA	Ultraviolet LED modules for Ca indicator and Light-regulated GABAA receptor (LIGABAR)

Features	PlexBright Table-top Module	PlexBright Compact Module	Remarks
Optical channels	1	1
Driver compatibility	– PlexBright 4 Channel Optogenetic Controller with Radiant Software, and – PlexBright LD-1 Single Channel LED Driver	– PlexBright 4 Channel OptogeneticController with Radiant Software, and – PlexBright LD-1 Single Channel LED Driver
Commutator compatibility	N/A	– PlexBright Dual LED Commutator, – PlexBright Dual LED +16 Channel Commutator for simultaneous use with analog headstages and – Carousel™ Commutator for simultaneous use with digital headstages – PlexBright Dual LED + Swivel Commutator – Motorized Carousel Commutator
Optical patch cable connector	Metal, notched FC Connector	Lightweight LC Connector with coupler
Optical patch cable tip options	– Bare fiber, – LC Ferrule, or – FC Ferrule	– LC Ferrule – FC Ferrule
Dimensions	82.6 x 63.5 x 30.0 mm (L x W x D) 3.3 x 2.5 x 1.2 inches	45.0 x 22.5 mm (D x Ø) 2.8 x .90 inches
Weight	91g	11g
Accessories	– PlexBright Light Measurement Kit – PlexBright Series-Y BNC Cable	– PlexBright Light Measurement Kit – PlexBright Series-Y BNC Cable	The PlexBright Light Measurement Kit ensures accurate output measurements of the custom drawn, high performance PlexBright optical fiber throughout the light path (unlikely to be accurately read without this kit and the contained adaptors). The PlexBright Series-Y BNC Cable enables the simultaneous operation of two PlexBright LED modules from a single driver/controller output (channel).

PlexBright LED Optogenetics Power Output at Various Points Throughout the Light Path

With High Performance Patch Cables

Table-top and Compact LED optogenetics modules offer identical power output specifications for a single wavelength/color.

Most information provided by other companies will report the highest possible output power – often directly from the LED itself. Plexon is more concerned with ensuring that researchers are clearly aware of practical output. The table below provides the expected system output actually experienced by the tissue – accounting for the specific wavelength chosen, loss due to patch cable and fiber stub connections, and the Fiber Stub Implant optical fiber diameter.

*** Important Power Output Note ** Plexon is committed to ensuring that researchers have the most accurate data available regarding the equipment used for their research. Therefore, we have taken extra care to provide the typical power outputs that are actually experienced at the tip of a 1.0m PlexBright Optical Patch Cable attached to a PlexBright LED Module. Other companies typically present the power output from the module itself, which represents the highest value possible. However, a more realistic number is that of the power output from the tip of an attached optical patch cable of appropriate fiber size, The information below represents the more realistic performance – and is only achievable if using the PlexBright high performance products. Any substitutions will not yield the high powers reported below. If comparing PlexBright products to those of other companies, be certain to verify if the power output reported is directly from the LED module, or the LED module plus 1.0m optical patch cable.

LED Module	At the LED Module	At the tip of a 200/230µm, 0.66NA Patch Cable*	At the tip of a 200/230µm, 0.66NA Fiber Stub or Magnetic Fiber Stub**	At the tip of a 110/125µm, 0.66NA Fiber Stub***
Color (Wavelength)	Measured Output¹	Measured Output¹ (Normalized Output)	Measured Output¹ (Normalized Output)	Measured Output¹ (Normalized Output)
Royal (450nm)	44.9mW	28.1mW (894mW/mm²)	22.5mW (715mW/mm²)	6.9mW (724mW/mm²)
Blue (465nm)	40.2mW	24.9mW (792mW/mm²)	19.9mW (634mW/mm²)	6.1mW (645mW/mm²)
Green (525nm)	11.8mW	7.8mW (249mW/mm²)	6.2mW (199mW/mm²)	1.9mW (201mW/mm²)
Lime (550nm)	19.8mW	11.5mW (336mW/mm²)	9.2mW (293mW/mm²)	2.8mW (295mW/mm²)
Yellow (590nm)	5.8mW	3.2mW (102mW/mm²)	2.6mW (82mW/mm²)	0.8mW (83mW/mm²)
Orange (620nm)	17.5mW	11.0mW (349mW/mm²)	8.8mW (279mW/mm²)	2.7mW (283mW/mm²)
Red (630nm)	19.5mW	11.8mW (375mW/mm²)	9.4mW (300mW/mm²)	2.9mW (304mW/mm²)
Crimson (660nm)	22.8mW	15.1mW (476mW/mm²)	12.0mW (381mW/mm²)	3.7mW (386mW/mm²)
Infrared 1 (850nm)	21.3mW	13.2mW (422mW/mm²)	10.6mW (337mW/mm²)	3.2mW (342mW/mm²)
Infrared 2 (940nm)	21.5mW	14.0mW (445mW/mm²)	11.2mW (356mW/mm²)	3.4mW (360mW/mm²)
Near-Infrared 1 (740nm)	12.3mW	8.5mW (270mW/mm²)	6.8mW (216mW/mm²)	2.1mW (219mW/mm²)
Near-Infrared 2 (760nm)	14.7mW	11.2mW (356mW/mm²)	8.9mW (285mW/mm²)	2.7mW (288mW/mm²)
Near-Infrared 3 (780nm)	11.0mW	7.2mW (228mW/mm²)	5.7mW (182mW/mm²)	1.8mW (184mW/mm²)
Near-Infrared 4 (810nm)	11.7mW	8.4mW (268mW/mm²)	6.7mW (215mW/mm²)	2.1mW (217mW/mm²)
Ultraviolet 1 (365nm)	28.1mW	13.5mW (430mW/mm²)	10.8mW (344mW/mm²)	3.3mW (348mW/mm²)
Ultraviolet 2 (385nm)	42.0mW	26.0mW (829mW/mm²)	20.8mW (663mW/mm²)	6.4mW (672mW/mm²)
Ultraviolet 3 (405nm)	41.8mW	27.2mW (865mW/mm²)	21.7mW (692mW/mm²)	6.7mW (701mW/mm²)

*Measured at the tip of a PlexBright High Performance Optical Patch Cable with a 200/230µm fiber 1.0m long.
**Measured at the tip of a PlexBright 200/230µm fiber diameter Fiber Stub Implant or 225/245µm fiber diameter magnetic fiber stub implant connected to a PlexBright High Performance Optical Patch Cable with a 200/230µm fiber 1.0m long.
***Measured at the tip of a PlexBright 110/125µm fiber diameter Fiber Stub Implant connected to a PlexBright High Performance Optical Patch Cable with a 200/230µm fiber 1.0m long.

¹Power values are measured using pulsed stimulation (1 msec pulses)

A Plexon Sales Engineer can help you determine the best optogenetic system configuration to meet your research goals.

With High Durability Patch Cables

Table-top and Compact LED modules offer identical power output specifications for a single wavelength/color.

LED Module	At the LED Module	At the tip of a 200/230µm, 0.5NA Patch Cable*	At the tip of a 200/230µm, 0.66NA Fiber Stub or Magnetic Fiber Stub**	At the tip of a 110/125µm, 0.66NA Fiber Stub***
Color (Wavelength)	Measured Output¹	Measured Output¹ (Normalized Output)	Measured Output¹ (Normalized Output)	Measured Output¹ (Normalized Output)
Royal (450nm)	44.9mW	21.8mW (694mW/mm²)	17.4mW (554mW/mm²)	5.4mW (569mW/mm²)
Blue (465nm)	40.2mW	19.0mW (605mW/mm²)	15.2mW (484mW/mm²)	4.7mW (495mW/mm²)
Green (525nm)	11.8mW	5.8mW (185mW/mm²)	4.6mW (146mW/mm²)	2.4mW (253mW/mm²)
Lime (550nm)	19.8mW	10.1mW (321mW/mm²)	8.1mW (258mW/mm²)	2.5mW (263mW/mm²)
Yellow (590nm)	5.8mW	3.0mW (95mW/mm²)	2.4mW (76mW/mm²)	0.8mW (84mW/mm²)
Orange (620nm)	17.5mW	9.2mW (293mW/mm²)	7.4mW (236mW/mm²)	2.3mW (242mW/mm²)
Red (630nm)	19.5mW	9.7mW (309mW/mm²)	7.7mW (245mW/mm²)	2.4mW (253mW/mm²)
Crimson (660nm)	22.8mW	11.5mW (366mW/mm²)	9.1mW (290mW/mm²)	2.8mW (295mW/mm²)
Infrared 1 (850nm)	21.3mW	10.7mW (341mW/mm²)	8.6mW (274mW/mm²)	2.6mW (274mW/mm²)
Infrared 2 (940nm)	21.5mW	10.4mW (331mW/mm²)	8.3mW (264mW/mm²)	2.5mW (263mW/mm²)
Near-Infrared 1 (740nm)	12.3mW	6.5mW (206mW/mm²)	5.2mW (165mW/mm²)	1.6mW (163mW/mm²)
Near-Infrared 2 (760nm)	14.7mW	7.8mW (250mW/mm²)	6.3mW (200mW/mm²)	1.9mW (198mW/mm²)
Near-Infrared 3 (780nm)	11.0mW	5.7mW (180mW/mm²)	4.5mW (144mW/mm²)	1.4mW (143mW/mm²)
Near-Infrared 4 (810nm)	11.7mW	6.1mW (195mW/mm²)	4.9mW (325mW/mm²)	1.5mW (154mW/mm²)
Ultraviolet 1 (365nm)	28.1mW	15.4mW (489mW/mm²)	12.3mW (391mW/mm²)	3.7mW (388mW/mm²)
Ultraviolet 2 (385nm)	42.0mW	23.0mW (732mW/mm²)	18.4mW (586mW/mm²)	5.5mW (581mW/mm²)
Ultraviolet 3 (405nm)	41.8mW	22.5mW (717mW/mm²)	18.0mW (573mW/mm²)	5.4mW (569mW/mm²)

*Measured at the tip of a PlexBright High Durability Optical Patch Cable with a 200/230µm fiber 1.0m long.

**Measured at the tip of a PlexBright 200/230µm fiber diameter Fiber Stub Implant or 225/245µm fiber diameter magnetic fiber stub implant connected to a PlexBright High Durability Optical Patch Cable with a 200/230µm fiber 1.0m long.

***Measured at the tip of a PlexBright 110/125µm fiber diameter Fiber Stub Implant connected to a PlexBright High Durability Optical Patch Cable with a 200/230µm fiber 1.0m long.

¹Power values are measured using pulsed stimulation (1 msec pulses)

A Plexon Sales Engineer can help you determine the best optogenetic system configuration to meet your research goals.