PlexBright Fiber Stub Implants

To complement the PlexBright® Optical Patch Cables, Plexon has developed an equally high performance line of PlexBright Fiber Stub Implants supporting chronic experiments for optogenetic stimulation. Our Standard Fiber Stubs are available in two ferrule sizes, two fiber diameters and a range of lengths. The NEW Magnetic Fiber Stubs and Lambda-B Fiber Stubs are available with LC ferrules and in a range of lengths.

A PlexBright Fiber Stub Implant is a hollow, ceramic ferrule (or cannula) with an optical fiber at the center that extends a specified length beyond one side of the ferrule. The fiber stub is permanently implanted into the head of the subject with the exposed fiber placed into the tissue. The standard Fiber Stubs are then connected to a PlexBright Optical Patch Cable using a ceramic sleeve during experimentation.

**New Magnetic Fiber Stubs  and  Lambda-B Fiber Stubs are now available**

Ferrule Tip Options
Standard PlexBright Fiber Stub Implants are available in two ferrule sizes: LC or FC Ferrules.  LC Ferrules are smaller (1.25mm OD x 6.45mm length) and used with either rats, mice or other small animals. FC Ferrules are a bit larger (2.5mm OD x 10.5mm length) and typically used with rats and other larger animals. Preference is strictly up to the researcher and the experimental conditions, with the above information offered as a loose guide.

**It is important that the ferrule size on the Fiber Stub Implant matches the ferrule tip on the Optical Patch Cable.  For example, if the subject is a rat and you choose an FC Ferrule Fiber Stub Implant, then be certain to also choose a PlexBright Optical Patch Cable with an FC Ferrule tip.**

Fiber Diameters
Standard PlexBright Fiber Stub Implants

These fiber stubs use the same high performance optical fiber that is found in our PlexBright Optical Patch Cables, and are manufactured in two diameters: our original 200/230µm fiber and the smaller 110/125µm fiber. The diameter measurements represent a core fiber diameter (200 or 110μm), and a total outer diameter that includes the cladding (230 or 125μm). Both sizes of PlexBright Fiber Stub Implants feature all-glass fibers and cladding – making them heat, chemical, and scratch resistant. The tips are also precision-finished for a perfect flat tip, without imperfections from cleaving that are typical in products from other companies.

There are a few considerations when choosing the right fiber size: size of animal, impact of tissue displacement, and required power output delivered to the tissue. Light output is a result of several factors including fiber diameter. If achieving the highest power output possible is of greatest concern, the original PlexBright Fiber Stub Implants measuring 200/230μm may be the most suitable. The smaller diameter fiber displaces less tissue upon entry and is believed to introduce less tissue damage. Thus, the smaller size may be more appropriate when minimal tissue disturbance is of special concern and/or for small animals such as mice.

**Before making a decision regarding the fiber diameter, reference the “Power Output at Tissue Interface Tables” on the Technical Specs tab. These tables provide an expected system output actually experienced by the tissue accounting for the specific wavelength chosen, loss due to patch cable and fiber stub connections, and the fiber stub fiber diameter.**

Lengths
The Fiber Stub Implant length refers to the length of the optical fiber exposed beyond the end of the ferrule (or cannula). PlexBright Fiber Stub Implants are available in a choice of 1, 2, 3, 4, 6, 8, 10 and 12mm lengths, or may be customized to the researcher’s specifications. The Fiber Stub Implants are sold in boxes of five or twenty five stubs.

To best maintain the PlexBright Fiber Stub Implant tip, Plexon recommends regular care and maintenance utilizing the PlexBright Optical Fiber Cleaning Kit.  This kit has been designed to safely maintain and extend the life of the exposed optical fiber.

PlexBright Starter Kits offer the initial products to get started and a 10% discount when purchased as a kit. Kits are available in four experimental designs and with either driver (the economical kit with the LD-1 Single Channel LED Driver or the fully functional kit with the 4 Channel Controller with Radiant™ Software). Starter Kit details are available on the PlexBright Quote Request Forms.

In addition to the PlexBright Fiber Stub Implants, tip-specific PlexBright Optical Patch Cables, and the PlexBright Optical Fiber Cleaning Kit, the PlexBright Optogenetic Stimulation System offers a complete range of super intensity PlexBright LED Modules – controlled by either the stand-alone PlexBright LD-1 Single Channel LED Driver or the advanced PlexBright 4 Channel Optogenetic Controller running Radiant Software, several PlexBright compatible commutators, and the PlexBright Light Measurement Kit. If your experiments include freely behaving animals, you may also be interested in learning more about the CinePlex® and CineLyzer® Behavioral Research Systems.

Your Plexon Sales Engineer is available to provide additional information and to assist you in determining what products might be the best choice for supporting your research.

Technical specifications for the Fiber Stub Implants may be found below, followed by a table detailing the light output actually experienced by the tissue during experimentation.

Technical specifications for the Fiber Stub Implants may be found below, followed by a table detailing the light output actually experienced by the tissue during experimentation.

PlexBright Quote Request Forms (specific to the experimental set-up) are available for rapid quote return and include both the Starter Kit itemization and individual parts for selection. The forms are available on the Resources tab, as well as on the Documentation page under the PlexBright header.

Features Standard
Fiber Stub
LC Ferrule Magnetic
Fiber Stub
Remarks
Lengths 1, 2, 3, 4, 6, 8, 10 or 12mm 1, 2, 3, 4, 6, 8, 10 or 12mm PlexBright Fiber Stub Implant length is the length of exposed fiber.  Lengths listed are standard and available immediately.  Custom options may also be available, and accordingly will require additional manufacturing time and cost.
Fiber diameters – 110/125µm, or
– 200/230µm
– 225/245µm The first number represents the core fiber diameter, while the second gives the total fiber diameter including the cladding. The larger fiber may be more advantageous if maximum power delivered to the tissue is especially important, while the smaller diameter fiber may be more appropriate when minimal tissue disturbance is of special concern, and/or when working with smaller animals such as mice.
Ferrule style LC Ferrule or FC Ferrule LC Ferrule
Ferrule OD
LC Ferrule: 1.25mm
FC Ferrule: 2.5mm
1.25mm
Ferrule length
LC Ferrule: 6.45mm

FC Ferrule: 10.5mm

LC Ferrule, Magnet, and Stainless Steel Tubing: 10.2mm
PlexBright Optical Patch Cable/Fiber Stub connection
LC Ceramic Sleeve
FC Ceramic Sleeve
Not required A bag of 5 ceramic sleeves come with the PlexBright Optical Patch Cable with an LC or FC Ferrule tip.
Packaging Box of 5 or 25 Box of 5 or 25
Accessories – PlexBright Light Measurement Kit
– PlexBright Optical Fiber Cleaning Kit
– PlexBright Light Measurement Kit
– PlexBright Optical Fiber Cleaning Kit
The PlexBright Light Measurement Kit ensures accurate output measurements of the custom drawn, high performance PlexBright optical fiber throughout the light path (unlikely to be accurately read without this kit and the contained adaptors).

The PlexBright Optical Fiber Cleaning Kit maximizes light transmission at fiber connections and extends the life of the fiber connection tips. It is recommended with the purchase of any of our PlexBright LED Modules, Optical Patch Cables and/or Fiber Stub Implants.

PlexBright LED Power Output at Various Points Throughout the Light Path

                               With High Performance Patch Cables 

LED Module At the
LED Module
At the tip of a 200/230µm, 0.66NA Patch Cable** At the tip of a 200/230µm, 0.66NA Fiber Stub*** At the tip of a     110/125µm, 0.66NA
Fiber Stub****
Color
(Wavelength)
Measured Output Measured Output
(Normalized Output)
Measured Output
(Normalized Output)
Measured Output
(Normalized Output)
Royal
(450nm)
44.9mW

28.1mW
(894mW/mm2)
22.5mW
(715mW/mm2)
6.9mW
(724mW/mm2)
Blue
(465nm)
40.2mW
24.9mW
(792mW/mm2)
19.9mW
(634mW/mm2)
6.1mW
(645mW/mm2)
Green
(525nm)
11.8mW

7.8mW
(249mW/mm2)
6.2mW
(199mW/mm2)
1.9mW
(201mW/mm2)
Lime
(550nm)
19.8mW

11.5mW
(336mW/mm2)
9.2mW
(293mW/mm2)
2.8mW
(295mW/mm2)
Yellow
(590nm)
5.8mW

3.2mW
(102mW/mm2)
2.6mW
(82mW/mm2)
0.8mW
(83mW/mm2)
Orange
(620nm)
17.5mW

11.0mW
(349mW/mm2)
8.8mW
(279mW/mm2)
2.7mW
(283mW/mm2)
Red
(630nm)
19.5mW

11.8mW
(375mW/mm2)
9.4mW
(300mW/mm2)
2.9mW
(304mW/mm2)
Crimson
(660nm)
22.8mW

15.1mW
(476mW/mm2)
12.0mW
(381mW/mm2)
3.7mW
(386mW/mm2)
Infrared 1
(850nm)
21.3mW

13.2mW
(422mW/mm2)
10.6mW
(337mW/mm2)
3.2mW
(342mW/mm2)
Infrared 2
(940nm)
21.5mW

14.0mW
(445mW/mm2)
11.2mW
(356mW/mm2)
3.4mW
(360mW/mm2)
Near-Infrared 1
(740nm)
12.3mW
8.5mW
(270mW/mm2)
6.8mW
(216mW/mm2)
2.1mW
(219mW/mm2)
Near-Infrared 2
(760nm)
14.7mW 11.2mW
(356mW/mm2)
8.9mW
(285mW/mm2)
2.7mW
(288mW/mm2)
Near-Infrared 3
(780nm)
11.0mW 7.2mW
(228mW/mm2)
5.7mW
(182mW/mm2)
1.8mW
(184mW/mm2)
Near-Infrared 4
(810nm)
11.7mW 8.4mW
(268mW/mm2)
6.7mW
(215mW/mm2)
2.1mW
(217mW/mm2)
Ultraviolet 1
(365nm)
28.1mW 13.5mW
(430mW/mm2)
10.8mW
(344mW/mm2)
3.3mW
(348mW/mm2)
Ultraviolet 2
(385nm)
42.0mW 26.0mW
(829mW/mm2)
20.8mW
(663mW/mm2)
6.4mW
(672mW/mm2)
Ultraviolet 3
(405nm)
41.8mW 27.2mW
(865mW/mm2)
21.7mW
(692mW/mm2)
6.7mW
(701mW/mm2)

*Measured at the tip of a PlexBright High Performance Optical Patch Cable with a 200/230µm fiber 1.0m long.
**Measured at the tip of a PlexBright 200/230µm fiber diameter Fiber Stub Implant connected to a PlexBright High Performance Optical Patch Cable with a 200/230µm fiber 1.0m long.
***Measured at the tip of a PlexBright 110/125µm fiber diameter Fiber Stub Implant connected to a PlexBright High Performance Optical Patch Cable with a 200/230µm fiber 1.0m long.

PlexBright LED Power Output at Various Points Throughout the Light Path

                               With High Durability Patch Cables 

LED Module At the
LED Module
At the tip of a 200/230µm, 0.5NA Patch Cable** At the tip of a 200/230µm, 0.5NA Fiber Stub*** At the tip of a     110/125µm, 0.5NA
Fiber Stub****
Color
(Wavelength)
Measured Output Measured Output
(Normalized Output)
Measured Output
(Normalized Output)
Measured Output
(Normalized Output)
Royal
(450nm)
44.9mW

21.8mW
(694mW/mm2)
17.4mW
(554mW/mm2)
5.4mW
(569mW/mm2)
Blue
(465nm)
40.2mW
19.0mW
(605mW/mm2)
15.2mW
(484mW/mm2)
4.7mW
(495mW/mm2)
Green
(525nm)
11.8mW

5.8mW
(185mW/mm2)
4.6mW
(146mW/mm2)
2.4mW
(253mW/mm2)
Lime
(550nm)
19.8mW

10.1mW
(321mW/mm2)
8.1mW
(258mW/mm2)
2.5mW
(263mW/mm2)
Yellow
(590nm)
5.8mW

3.0mW
(95mW/mm2)
2.4mW
(76mW/mm2)
0.8mW
(84mW/mm2)
Orange
(620nm)
17.5mW

9.2mW
(293mW/mm2)
7.4mW
(236mW/mm2)
2.3mW
(242mW/mm2)
Red
(630nm)
19.5mW

9.7mW
(309mW/mm2)
7.7mW
(245mW/mm2)
2.4mW
(253mW/mm2)
Crimson
(660nm)
22.8mW

11.5mW
(366mW/mm2)
9.1mW
(290mW/mm2)
2.8mW
(295mW/mm2)
Infrared 1
(850nm)
21.3mW

10.7mW
(341mW/mm2)
8.6mW
(274mW/mm2)
2.6mW
(274mW/mm2)
Infrared 2
(940nm)
21.5mW

10.4mW
(331mW/mm2)
8.3mW
(264mW/mm2)
2.5mW
(263mW/mm2)
Near-Infrared 1
(740nm)
12.3mW
6.5mW
(206mW/mm2)
5.2mW
(165mW/mm2)
1.6mW
(163mW/mm2)
Near-Infrared 2
(760nm)
14.7mW 7.8mW
(250mW/mm2)
6.3mW
(200mW/mm2)
1.9mW
(198mW/mm2)
Near-Infrared 3
(780nm)
11.0mW 5.7mW
(180mW/mm2)
4.5mW
(144mW/mm2)
1.4mW
(143mW/mm2)
Near-Infrared 4
(810nm)
11.7mW 6.1mW
(195mW/mm2)
4.9mW
(325mW/mm2)
1.5mW
(154mW/mm2)
Ultraviolet 1
(365nm)
28.1mW 15.4mW
(489mW/mm2)
12.3mW
(391mW/mm2)
3.7mW
(388mW/mm2)
Ultraviolet 2
(385nm)
42.0mW 23.0mW
(732mW/mm2)
18.4mW
(586mW/mm2)
5.5mW
(581mW/mm2)
Ultraviolet 3
(405nm)
41.8mW 22.5mW
(717mW/mm2)
18.0mW
(573mW/mm2)
5.4mW
(569mW/mm2)

*Measured at the tip of a PlexBright High Durability Optical Patch Cable with a 200/230µm fiber 1.0m long.   

**Measured at the tip of a PlexBright 200/230µm fiber diameter Fiber Stub Implant connected to a PlexBright High Durability Optical Patch Cable with a 200/230µm fiber 1.0m long.

***Measured at the tip of a PlexBright 110/125µm fiber diameter Fiber Stub Implant connected to a PlexBright High Durability Optical Patch Cable with a 200/230µm fiber 1.0m long.

PlexBright LED Power Output With Head-Mounted LED Modules 

LED Module At the
LED Module
At the tip of a 200/230µm, 0.66NA Fiber Stub (No patch cable required)
Color
(Wavelength/Max Current)
Measured Output Measured Output
(Normalized Output)
Head-Mounted Blue
(465nm/300mA)
62.2mW
21.8mW
(693mW/mm2)
Head-Mounted Green
(525nm/300mA)
19.1mW

6.7mW
(213mW/mm2)
Head-Mounted Lime
(550nm/500mA)
33.3mW

11.7mW
(371mW/mm2)
Head-Mounted Orange
(620nm/250mA)
33.9mW

11.9mW
(378mW/mm2)

 

PlexBright LED Power Output With Magnetic Head-Mounted LED Modules 

LED Module At the
LED Module
At the tip of a Magnetic Fiber Stub (No patch cable required)
Color
(Wavelength/Max Current)
Measured Output Measured Output
(Normalized Output)
Magnetic Head-Mounted Blue
(465nm/300mA)
35.3mW 28.3mW
(712mW/mm2)
Magnetic Head-Mounted Green
(525nm/300mA)
12mW 9.6mW
(241mW/mm2)
Magnetic Head-Mounted Lime
(550nm/500mA)
14mW 11.2mW
(322mW/mm2)
Magnetic Head-Mounted Orange
(620nm/250mA)
13mW 10.4mW
(362mW/mm2)

 

Most information provided by other companies will report the highest possible output power – often directly from the LED itself. Plexon is more concerned with ensuring that researchers are clearly aware of practical output. The table below provides the expected system output actually experienced by the tissue – accounting for the specific wavelength chosen, loss due to patch cable and fiber stub connections, and the Fiber Stub Implant optical fiber diameter.

 

If you have further questions, a Plexon Sales Engineer will be happy to help.