Dual-Wavelength Commutator-Based Photometry System

The Dual-Wavelength Photometry System incorporates new features based on customer feedback.

The Plexon Commutator Photometry System is a dual-wavelength camera-based system for use with freely-behaving animals. A branching patch cable enables simultaneous recording up to four different regions. The system stimulates with blue (465nm) light and uses UV (410nm) light as an isosbetic control signal. The fluorescence is measured with a camera, which allows up to four fibers to be measured at the same time.

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Dual-Wavelength Commutator-Based Photometry System

The Dual-Wavelength Photometry System incorporates new features based on customer feedback.

The Plexon Commutator Photometry System is a dual-wavelength camera-based system for use with freely-behaving animals. A branching patch cable enables simultaneous recording up to four different regions. The system stimulates with blue (465nm) light and uses UV (410nm) light as an isosbetic control signal. The fluorescence is measured with a camera, which allows up to four fibers to be measured at the same time.

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Dual-Wavelength Commutator-Based Photometry System

This camera-based system with commutator includes two built in LEDs for excitation and one optical sensor which allow for detection of green fluorescence and a branching patch cable provides the user with the ability to record from multiple brain regions. The system interleaves the 410 nm and 465 nm LEDS and the camera detecting green fluorescence captures light for each of these wavelengths independently, which allows the user to identify the calcium-dependent fluorescence (isosbestic control).

System Features

  • The Inertial Measurement Unit IMU commutator enables smooth, artifact-free rotation with no optical joint
  • Capture neural activity in freely behaving animals and minimizes degradation and tethering constraints
  • LED scheduler reduces risk of photobleaching by configuring ON/OFF schedule for LEDS
  • Record from multiple locations in a single animal
  • Detect fluorescence at 525 nm using one detection camera
  • Align photometry signals with behavioral events using digital inputs
  • Integrated video tracking software

465 nm for selectively activating GCaMP6

410 nm for use as an isosbestic control to detect calcium-independent signals

 

Dual-Wavelength 4-fiber Commutator-Based Photometry System

This camera-based system with commutators includes two built in LEDs for excitation and one optical sensor which allow for detection of green fluorescence and a branching patch cable provides the user with the ability to record from multiple brain regions or multiple animals. The system interleaves the 410 nm and 465 nm LEDS and the camera detecting green fluorescence captures light for each of these wavelengths independently, which allows the user to identify the calcium-dependent fluorescence (isosbestic control).

System Features

  • The Inertial Measurement Unit IMU commutator enables smooth, artifact-free rotation with no optical joint
  • Capture neural activity in freely behaving animals and minimizes degradation and tethering constraints
  • LED scheduler reduces risk of photobleaching by configuring ON/OFF schedule for LEDS
  • Record from multiple locations in a single animal
  • Detect fluorescence at 525 nm using one detection camera
  • Align photometry signals with behavioral events using digital inputs
  • Integrated video tracking software

 

465 nm for selectively activating GCaMP6

410 nm for use as an isosbestic control to detect calcium-independent signals