A tutorial on how to setup Plexon’s Cinelyzer with Multi-Fiber Photometry System. Plexon’s Multi-Fiber Photometry System is a camera based system that images one end of a branching patch cable containing multiple fibers and extracts the photometry signal from the individual fibers in the bundle via image processing techniques.

One of the major hurdles for a lab performing fiber photometry is data analysis. Using and applying an isosbestic control requires a complex set of mathematics and understanding how to do this correctly is difficult for someone just starting with the technique. View all 4 parts here.

pMAT vs GuPPY: One of the major hurdles for a lab performing fiber photometry is data analysis. Using and applying an isosbestic control requires a complex set of mathematics and understanding how to do this correctly is difficult for someone just starting with the technique. Perhaps the barrier is a lack of coding experience in the lab, which makes it difficult to apply the complex calculations to a set of data. Or the reason could even be that it is unclear which analyses are appropriate for fiber photometry data. Whatever the reason, it is becoming apparent to the research community that a standard method needs to be set.