New Release:

Multi-Wavelength Multi-Fiber Photometry

The new multi-wavelength photometry system builds on the technology of the legacy single-wavelength photometry system and incorporates new features based on customer feedback.

This camera-based system includes three built in LEDs for excitation and two optical sensors, which allow for detection of green and red fluorescence simultaneously. A branching patch cable provides the user with the ability to record from multiple brain regions or multiple animals.

The system interleaves the 410 nm and 465 nm LEDS and the camera detecting green fluorescence captures light for each of these wavelengths independently, which allows the user to identify the calcium-dependent fluorescence (isobestic control).

Features

• Record from multiple locations in a single animal or many animals simultaneously with a single system
• Three excitation ranges centered at 560 nm, 465 nm, and 410 nm
• Detect fluorescence at 525 nm and 600 nm using two detection cameras
• Synchronize photometry signals with behavioral events
• Incorporated
  LED driver
• Integrated with video tracking software

Tracking and Data Analysis Software

• Visualize raw photometry data and ΔF/F in real-time
• Heatmaps for each individual fiber illustrate changes in fluorescence during recording
• Create zones and automatically count the number of behavioral events that occur during recording
• Define photometry events based on ΔF/F threshold crossings and combine with behavioral
  events to identify if a change in florescence occurs during a specified behavior
• Interface with External Equipment
• Track speed and position of subject

System Diagram