CinePlex 3D Option
CinePlex® 3D is one of three advanced application-specific options to enhance the CinePlex Behavioral Research System. The CinePlex 3D option empowers the recording and tracking of color markers from multiple video streams in three dimensions – all with remarkably simple system and camera calibration.
**CinePlex Studio 3.11.0 now offers jitter elimination and more!**
CinePlex 3D is an optional program with CinePlex Studio. It takes the advanced, two-dimensional tracking functionality available through CinePlex Tracking to the next level. The specialized capabilities unleashed through CinePlex 3D can be grouped into two primary topics: the obvious, yet remarkable, ability to track an animal’s movements in three dimensions, and the brilliant simplicity of RapidGrid™ camera and system calibration, no longer requiring a dedicated environment for video capture.
Three-dimensional tracking requires a minimum of two cameras. However, CinePlex 3D is capable of handling up to four simultaneously recording video feeds. The optimal number of cameras depends on the complexity of the experimental arena and the objects’ expected movements.
Placement of the cameras is exceptionally flexible allowing for various overhead, as well as broadside, positions. Up to 12 colored markers can be simultaneously tracked generating three-dimensional reconstructions of movement using the projections as captured from the multiple cameras. For example, markers can be placed at strategic locations on a monkey’s leg or arm to capture movement metrics.
In a typical recording environment supporting three-dimensional behavioral research, cameras are permanently affixed to specific locations in a dedicated recording chamber. This rigid design is primarily due to the extreme complexity and extensive effort required to perform the system’s intricate calibration. As a result, options are limited, and funds that could have been directed elsewhere are sunk into a single-purpose, immovable recording facility. CinePlex 3D changes the game altogether.
CinePlex 3D embeds a highly sophisticated – yet easy to execute – RapidGrid protocol within the program to reduce calibration time to only minutes per camera. Researchers are free to establish experiments wherever they deem appropriate – any room, building or facility most conducive to eliciting the results desired for their immediate purpose. CinePlex 3D’s almost “mobile” approach redefines the possibilities for the behaviorist.
Also working with the OmniPlex® or MAP System online, tracking coordinates are also available to online user-written programs via MATLAB® and C/C++ software development kits (SDKs). The SDKs permit further automation of the data manipulation, reducing human error and time required to manually analyze the data.
CinePlex 3D is a specialized program within CinePlex Studio that builds on the CinePlex Tracking functionality and is not sold separately. To access the CinePlex 3D functionality, a special license is required.
You may also be interested in the other specialized application-specific options available within CinePlex Studio – CinePlex Basic Behavior. Beyond the CinePlex System, and if your freely behaving animal experiments involve neural data acquisition, other products such as our PlexBright® Optogenetic Stimulation System, commutators and specialized headstage cables with ultra-fine wire and/or protective wraps may be of value to you.
You are invited to contact a Plexon Sales Engineer for more information and to assist you in determining how to best configure the CinePlex System suite of programs to most effectively support your research.
CinePlex 3D performs advanced – yet optional – functionality within CinePlex Studio that builds upon the CinePlex Tracking. The table below outlines selected information regarding CinePlex 3D. Click here to learn more about CinePlex Tracking, or CinePlex Software in general.
|Features||Specifications and Options||Remarks|
|Coordinate dimensions||Three-dimensional (3D)|
|Light conditions accommodated||Daylight to dark environments||Calibration must be performed in normal light. Three-dimensional tracking operates under normal light and under near infrared conditions.|
|Number of cameras supported||2 to 4||See camera types on the CinePlex Software page.|
|Camera positioning||Overhead, underneath and broadside||Cameras are not limited to being positioned at angles over the animals, but can also be positioned around the sides or underneath the environments.|
|Calibration method||RapidGrid™ calibration||Once cameras are positioned, a calibration grid provided by Plexon is used to generate the frame of reference moving systematically from camera to camera. Calibration files can be reused until one or more cameras are moved.|
|Video capture environment locations||Practically unlimited and changeable from experiment to experiment, if desired.||Other than the requirement for the CinePlex System equipment to remain dry, the only limitations on location precipitate from the study requirements themselves. For example, if the research includes neural recording, then proximity to the neural data acquisition system is a limitation. The easy-to-execute calibration protocols free research from fixed, dedicated locations.|
|Tracking method||Color markers, or LED tracking||Up to 12 color markers can be tracked, or up to three LEDs.|
|Relationship to other CinePlex Studio application-specific options||Requires data captured from CinePlex Tracking.|
|Licensing||Requires the purchase of a CinePlex System, plus a license key for CinePlex Tracking and CinePlex 3D.||All upgrades within a software version are free of charge and do not need a modification to the license key. Upgrades to the next version do require an updated key with expanded privileges|
Any questions? Ask a Plexon Sales Engineer. We are here to help you explore whether the CinePlex System is the best tool to launch you from experiment to publication the fastest.
Post date December 2015
Post Date September 2018
Post date October 2014. This user guide contains updated functionality for the use of CinePlex integrated with OmniPlex only.
Legacy Manuals/User Guides
Post date February 2010. Comprehensive CinePlex User Guide through version 3.0.
Post date February 2008.
Post date November 2021
Post date May 2014
Post date August 2014
Guides and How To Papers
Technical Specs and Data Sheets
Research Articles with Video
Jacobson, Tara K., Jonathan W. Ho, Brendon W. Kent, Fang-Chi Yang, and Rebecca D. Burwell. “Automated Visual Cognitive Tasks for Recording Neural Activity Using a Floor Projection Maze.” JoVE (Journal of Visualized Experiments) 84 (2014): e51316-e51316.