Quartet: Calcium Imaging in Freely Moving Animals

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Quartet: Calcium Imaging in Freely Moving Animals

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Quartet is a miniature fluorescence microscope and data collection software package optimized for neuronal calcium imaging.

Thin, cylindrical, graded-refractive-index lenses (GRIN) get implanted along with our specially designed, patent-pending focus adjustable fiber-lens connector in ANY region of the brain and spinal cord.

The imaging fiber bundle connects to the implanted GRIN lens to transmit the fluorescence signal from neurons to the CMOS sensor. Quartet uses FOUR optical imaging fiber bundles and comes with four such highly flexible and light weight lens connectors. Each bundle includes 18000 single fibers, each of which are 7.5micron in diameter.

The design of the connector is such that even when the fiber probe is disconnected the initially set focus remains the same. This ensures the same plane of neurons is imaged as days go by, ensuring any observed changes come from the changes in the neuronal micro-circuitry and not from focusing on a different plane of neurons.

We currently offer two types of GRIN lenses for imaging different areas of the central nervous system. The first lens option is designed to reach any area of the brain in rodents or larger animals and measures 0.7mm in diameter and more than 8mm long. The second lens options is 1mm in diameter and can reach areas in the brain which are up to 2.5mm deep.

This images shows the size of the connector and grin lens relative to an American dime.


Quartet is a miniature fluorescence microscope and data collection software package optimized for neuronal calcium imaging.

Thin, cylindrical, graded-refractive-index lenses (GRIN) get implanted along with our specially designed, patent-pending focus adjustable fiber-lens connector in ANY region of the brain and spinal cord.

The imaging fiber bundle connects to the implanted GRIN lens to transmit the fluorescence signal from neurons to the CMOS sensor. Quartet uses FOUR optical imaging fiber bundles and comes with four such highly flexible and light weight lens connectors. Each bundle includes 18000 single fibers, each of which are 7.5micron in diameter.

The design of the connector is such that even when the fiber probe is disconnected the initially set focus remains the same. This ensures the same plane of neurons is imaged as days go by, ensuring any observed changes come from the changes in the neuronal micro-circuitry and not from focusing on a different plane of neurons.

We currently offer two types of GRIN lenses for imaging different areas of the central nervous system. The first lens option is designed to reach any area of the brain in rodents or larger animals and measures 0.7mm in diameter and more than 8mm long. The second lens options is 1mm in diameter and can reach areas in the brain which are up to 2.5mm deep.

This images shows the size of the connector and grin lens relative to an American dime.

Plexon is an authorized distributor of Quartet

Much Smaller than Comparable Devices

Quartet

Others

Microscope is not mounted on the animals head allowing for greater flexibility in experimental design.

This design makes it easier to combine calcium imaging with Optogenetics, electrophysiology, and fMRI

Much Smaller than Comparable Devices

Neurescence

Others

Microscope is not mounted on the animals head allowing for greater flexibility in experimental design.

This design makes it easier to combine calcium imaging with Optogenetics, electrophysiology, and fMRI

Easy Connection & Lens Focus-Lock

Small, easy to use fiber/lens connection with Neurescence's proprietary focus-lock capability

The design of the connector is such that even when the fiber probe is disconnected the initially set focus remains the same. This ensures the same plane of neurons is imaged as days go by, ensuring any observed changes come from the changes in the neuronal micro-circuitry and not from focusing on a different plane of neurons.

Freedom to Use in Any Behavioral Paradigm

• Highly flexible fibers that minimize interference with animal movement.

Electronics are located far from the head of the animal, which allows for experiments such as the water maze and the forced swim test

Record from multiple animals simultaneously during social interaction experiments

Accessories


Fiber Optic Cables

Lens-Connectors

Piccolo - Surgery Assist Device

Easy Connection & Lens Focus-Lock

Small, easy to use fiber/lens connection with Neurescence's proprietary focus-lock capability

The design of the connector is such that even when the fiber probe is disconnected the initially set focus remains the same. This ensures the same plane of neurons is imaged as days go by, ensuring any observed changes come from the changes in the neuronal micro-circuitry and not from focusing on a different plane of neurons.

Freedom to Use in Any Behavioral Paradigm

• Highly flexible fibers that minimize interference with animal movement.

Electronics are located far from the head of the animal, which allows for experiments such as the water maze and the forced swim test

Record from multiple animals simultaneously during social interaction experiments

Accessories


Fiber Optic Cables

Lens-Connectors

Piccolo - Surgery Assist Device