Chromatone

Simultaneous multi-color imaging and multi-opsin stimulation in up to four brain areas in freely behaving animals

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Chromatone: Simultaneous Multi-color Multi-region Functional Imaging

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Chromatone System

The Chromatone™ system is the only complete solution for in vivo calcium imaging and optogenetics, that enables simultaneous multicolor imaging and multi-opsin stimulation in up to four brain or spinal cord areas in freely behaving animals.

• Simultaneously image and modulate neuronal activity in four areas of brain or spinal cord.
• GCaMP, tdTomato imaging and ChrimsonR optogenetic stimulation.
• Each color can be controlled to be emitted as a continuous wave for imaging and as pulses with various duty cycles for optogenetic stimulation.
• Emitting power level for each wavelength can be independently controlled in each fiber.
• Enables the simultaneous recording of neurons at cellular resolution

The components include the main microscope body, four illumination cubes, control electronics, multimode fibers and imaging fiber bundles. The microscope body is small and light weight, to ensure ease of use during a wide array of behavior paradigms and it integrates cell-type specific calcium imaging and optogenetics.

The illumination cubes contain excitation light sources and optical filters to ensure the correct illumination color arrives at the brain tissue. Active temperature control in the illumination cubes ensures highly stable illumination levels at the brain tissue, throughout a behavior session. Each illumination cube connects to the microscope body through a multimode fiber and emits three illumination colors:

  • 470nm +/- 12nm for Green Fluorescent Protein Imaging/ChannelRhodopsin optogenetics
  • 550nm +/-7.5nm for Red Fluorescent Protein imaging
  • 632nm +/- 14nm for ChrimsonR Optogenetics
Light from each illumination cube is coupled into an imaging fiber bundle, which carries the light to implanted GRIN lens connectors and then to the brain tissue.

There are four such imaging fiber bundles, enabling simultaneous multicolor calcium imaging and multi opsin stimulation in up to four brain or spinal cord areas.

During an imaging -behavior session, the imaging fibre bundles of the Chromatone™ are connected to the implanted lens-connectors. Three different illumination colors are available in each implanted region, to enable multicolor imaging (GFP and/or RFP) and multi-opsin optogenetics (ChannelRhodopsin / ChrimsonR). The three illumination colors red, blue and green in each implanted region can be independently controlled using the software.

Plexon is an authorized distributor of Chromatone™.


The Chromatone™ system is the only complete solution for in vivo calcium imaging and optogenetics, that enables simultaneous multi-color imaging and multi-opsin stimulation in up to four brain or spinal cord areas in freely behaving animals.

• Simultaneously image and modulate neuronal activity in four areas of brain or spinal cord.
• GCaMP, tdTomato imaging and ChrimsonR optogenetic stimulation.
• Each wavelength can be emitted continuously for imaging or pulsed (with various duty cycles) for optogenetic stimulation.
• Emitting power level for each wavelength can be independently controlled in each fiber.
• Enables the simultaneous recording of neurons at cellular resolution

The components include the main microscope body, four illumination cubes, control electronics, multimode fibers and imaging fiber bundles. The microscope body is small and light weight, to ensure ease of use during a wide array of behavior paradigms and it integrates cell-type specific calcium imaging and optogenetics.

The illumination cubes contain excitation light sources and optical filters to ensure the correct illumination color arrives at the brain tissue. Active temperature control in the illumination cubes ensures highly stable illumination levels at the brain tissue, throughout a behavior session. Each illumination cube connects to the microscope body through a multimode fiber and emits three illumination colors:

  • 470nm +/- 12nm for Green Fluorescent Protein Imaging/ChannelRhodopsin optogenetics
  • 550nm +/-7.5nm for Red Fluorescent Protein imaging
  • 632nm +/- 14nm for ChrimsonR Optogenetics
Light from each illumination cube is coupled into an imaging fiber bundle, which carries the light to implanted GRIN lens connectors and then to the brain tissue.

There are four such imaging fiber bundles, enabling simultaneous multicolor calcium imaging and multi opsin stimulation in up to four brain or spinal cord areas.

During an imaging -behavior session, the imaging fibre bundles of the Chromatone™ are connected to the implanted lens-connectors. Three different illumination colors are available in each implanted region, to enable multicolor imaging (GFP and/or RFP) and multi-opsin optogenetics (ChannelRhodopsin / ChrimsonR). The three illumination colors red, blue and green in each implanted region can be independently controlled using the software.

Plexon is an authorized distributor of Chromatone™.

Much Smaller than Comparable Devices

Neurescence

Others

Microscope is not mounted on the animal's head allowing for greater flexibility in experimental design.

This design makes it easier to combine calcium imaging with Optogenetics, electrophysiology, and fMRI

Much Smaller than Comparable Devices

The Chromatone microscope is not mounted on the animal's head allowing for greater flexibility in experimental design. In addition, there is less weight on the animal which results in more naturalistic behavior.

This design makes it easier to combine calcium imaging with Optogenetics, electrophysiology, and fMRI.

Image

Easy Connection & Lens Focus-Lock

Small, easy to use fiber/lens connection with Neurescence's proprietary focus-lock capability

The design of the connector is such that even when the fiber probe is disconnected the initially set focus remains the same. This ensures the same plane of neurons is imaged as days go by, ensuring any observed changes come from the changes in the neuronal micro-circuitry and not from focusing on a different plane of neurons.

Freedom to Use in Any Behavioral Paradigm

The highly flexible fibers minimize interference with animal movement and give researchers the freedom to use the system in almost any behavioral experiment. 

Electronics are located far from the head of the animal, which allows for experiments such as the water maze and the forced swim test. In addition, record from multiple animals simultaneously during social interaction experiments using the imaging fiber bundle that can used with up to 4 animals. 

Accessories


Piccolo - Surgery Assist Device

Easy Connection & Lens Focus-Lock

Small, easy to use fiber/lens connection with Neurescence's proprietary focus-lock capability

The design of the connector is such that even when the fiber probe is disconnected the initially set focus remains the same. This ensures the same plane of neurons is imaged as days go by, ensuring any observed changes come from the changes in the neuronal micro-circuitry and not from focusing on a different plane of neurons.

Freedom to Use in Any Behavioral Paradigm

• Highly flexible fibers that minimize interference with animal movement.

Electronics are located far from the head of the animal, which allows for experiments such as the water maze and the forced swim test

Record from multiple animals simultaneously during social interaction experiments

Accessories


Piccolo - Surgery Assist Device

Piccolo - Surgery Assist Device